Russian Journal
of Developmental Biology

Andrey G. Zaraisky

Personal information
He is currently head of the Laboratory of molecular bases of embryogenesis.

Education
Period Country, city Education institution Additional info
1990 Russia, Moscow M.V. Lomonosov Moscow State University (biological faculty) PhD in molecular biology
2000 Russia, Moscow M.V. Lomonosov Moscow State University (biological faculty) DSc in molecular biology and developmental biology
2011 Russia, Moscow professor in molecular biology

Scientific interests
The main scientific interests of Dr. Zaraisky are focused on structural and functional study of genes and proteins that regulate embryonic development of the brain.

Awards & honors
He is the winner of prizes, A.A. Baev (2001) and A.O. Kovalevsky, Russian Academy of Sciences (2006).

Main scientific results
Under the leadership of Dr. Zaraisky in the laboratory of molecular bases of embryogenesis a novel class of homeobox genes, Anf, was discovered and studied. These genes encode for the homeodomain transcription factors, which play a key role in regulation of the early forebrain development in Vertebrates. Further investigation of the gene network related to the functioning of Anf led to the discovery of a novel, the 8th, a family of small GTFases, named as Ras-dva, which are specifically expressed in the forebrain rudiment and regulate its early development. An important achievement of the laboratory of molecular bases of embryogenesis is discovering of two novel families of secreted proteins, Noggin2 and Noggin4, whose genes are controlled by Anf homeodomain factors. These novel representatives of Noggin proteins are able to bind and thereby inhibit a number of growth factors of the TGF-? superfamily.

Scientific societies’ membership
Dr. Zaraisky is a member of the Academic and Dissertation councils of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, Editorial Boards of the journals Molecular Biology and Russian Journal of Developmental Biology.

Selected publications

1. Ivanova AS, Korotkova DD, Ermakova GV, Martynova NY, Zaraisky AG, Tereshina MB (2018). Ras-dva small GTPases lost during evolution of amniotes regulate regeneration in anamniotes. Sci Rep 8 (1), 13035

2. Kotlobay AA, Sarkisyan KS, Mokrushina YA, Marcet-Houben M, Serebrovskaya EO, Markina NM, Gonzalez Somermeyer L, Gorokhovatsky AY, Vvedensky A, Purtov KV, Petushkov VN, Rodionova NS, Chepurnyh TV, Fakhranurova LI, Guglya EB, Ziganshin R, Tsarkova AS, Kaskova ZM, Shender V, Abakumov M, Abakumova TO, Povolotskaya IS, Eroshkin FM, Zaraisky AG, Mishin AS, Dolgov SV, Mitiouchkina TY, Kopantzev EP, Waldenmaier HE, Oliveira AG, Oba Y, Barsova E, Bogdanova EA, Gabaldon T, Stevani CV, Lukyanov S, Smirnov IV, Gitelson JI, Kondrashov FA, Yampolsky IV (2018). Genetically encodable bioluminescent system from fungi. Proc Natl Acad Sci U S A 115 (50), 12728–12732

3. Martynova NY, Parshina EA, Ermolina LV, Zaraisky AG (2018). The cytoskeletal protein Zyxin interacts with the zinc-finger transcription factor Zic1 and plays the role of a scaffold for Gli1 and Zic1 interactions during early development of Xenopus laevis. Biochem Biophys Res Commun 504 (1), 251–256

4. Martynova NY, Eroshkin FM, Оrlov EE, Zaraisky AG (2018). HMG-box factor SoxD/Sox15 and homeodomain-containing factor Xanf1/Hesx1 directly interact and regulate the expression of Xanf1/Hesx1 during early forebrain development in Xenopus laevis. Gene 638, 52–59

5. Eroshkin FM, Zaraisky AG (2017). Mechano-sensitive regulation of gene expression during the embryonic development. Genesis 55 (4),

6. Nesterenko AM, Kuznetsov MB, Korotkova DD, Zaraisky AG (2017). Morphogene adsorption as a Turing instability regulator: Theoretical analysis and possible applications in multicellular embryonic systems. PLoS One 12 (2), e0171212

7. Bayramov AV, Ermakova GV, Eroshkin FM, Kucheryavyy AV, Martynova NY, Zaraisky AG (2016). The presence of Anf/Hesx1 homeobox gene in lampreys suggests that it could play an important role in emergence of telencephalon. Sci Rep 6, 39849

8. Eroshkin FM, Nesterenko AM, Borodulin AV, Martynova NY, Ermakova GV, Gyoeva FK, Orlov EE, Belogurov AA, Lukyanov KA, Bayramov AV, Zaraisky AG (2016). Noggin4 is a long-range inhibitor of Wnt8 signalling that regulates head development in Xenopus laevis. Sci Rep 6, 23049

9. Nesterenko AM, Orlov EE, Ermakova GV, Ivanov IA, Semenyuk PI, Orlov VN, Martynova NY, Zaraisky AG (2015). Affinity of the heparin binding motif of Noggin1 to heparan sulfate and its visualization in the embryonic tissues. Biochem Biophys Res Commun 468 (12), 331–336

10. Matlashov ME, Bogdanova YA, Ermakova GV, Mishina NM, Ermakova YG, Nikitin ES, Balaban PM, Okabe S, Lukyanov S, Enikolopov G, Zaraisky AG, Belousov VV (2015). Fluorescent ratiometric pH indicator SypHer2: Applications in neuroscience and regenerative biology. BIOCHIM BIOPHYS ACTA 1850 (11), 2318–2328

11. Pereverzev AP, Gurskaya NG, Ermakova GV, Kudryavtseva EI, Markina NM, Kotlobay AA, Lukyanov SA, Zaraisky AG, Lukyanov KA (2015). Method for quantitative analysis of nonsense-mediated mRNA decay at the single cell level. Sci Rep 5, 7729

12. Ivanova AS, Shandarin IN, Ermakova GV, Minin AA, Tereshina MB, Zaraisky AG (2015). The secreted factor Ag1 missing in higher vertebrates regulates fins regeneration in Danio rerio. Sci Rep 5, 8123

13. Tereshina MB, Ermakova GV, Ivanova AS, Zaraisky AG (2014). Ras-dva1 small GTPase regulates telencephalon development in Xenopus laevis embryos by controlling Fgf8 and Agr signaling at the anterior border of the neural plate. Biol Open 3 (3), 192–200

14. Martynova NY, Ermolina LV, Ermakova GV, Eroshkin FM, Gyoeva FK, Baturina NS, Zaraisky AG (2013). The cytoskeletal protein Zyxin inhibits Shh signaling during the CNS patterning in Xenopus laevis through interaction with the transcription factor Gli1. Dev Biol 380 (1), 37–48

15. Ivanova AS, Tereshina MB, Ermakova GV, Belousov VV, Zaraisky AG (2013). Agr genes, missing in amniotes, are involved in the body appendages regeneration in frog tadpoles. Sci Rep 3, 1279

16. Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM, Shcherbo D (2012). A monomeric red fluorescent protein with low cytotoxicity. Nat Commun 3, 1204

17. Borodulin AV, Eroshkin FM, Bayramov AV, Zaraisky AG (2012). Noggin4 expression during chick embryonic development. Int J Dev Biol 56 (5), 403–406

18. Bayramov AV, Eroshkin FM, Martynova NY, Ermakova GV, Solovieva EA, Zaraisky AG (2011). Novel functions of Noggin proteins: Inhibition of Activin/Nodal and Wnt signaling. Development 138 (24), 5345–5356

19. Serebrovskaya EO, Gorodnicheva TV, Ermakova GV, Solovieva EA, Sharonov GV, Zagaynova EV, Chudakov DM, Lukyanov S, Zaraisky AG, Lukyanov KA (2011). Light-induced blockage of cell division with a chromatin-targeted phototoxic fluorescent protein. Biochem J 435 (1), 65–71

20. Tereshina MB, Bayramov AV, Zaraisky AG (2011). Expression patterns of genes encoding small GTPases Ras-dva-1 and Ras-dva-2 in the Xenopus laevis tadpoles. Gene Expr Patterns 11 (12), 156–161

21. Shcherbo D, Shemiakina II, Ryabova AV, Luker KE, Schmidt BT, Souslova EA, Gorodnicheva TV, Strukova L, Shidlovskiy KM, Britanova OV, Zaraisky AG, Lukyanov KA, Loschenov VB, Luker GD, Chudakov DM (2010). Near-infrared fluorescent proteins. Nat Methods 7 (10), 827–829

22. Shcherbo D, Murphy CS, Ermakova GV, Solovieva EA, Chepurnykh TV, Shcheglov AS, Verkhusha VV, Pletnev VZ, Hazelwood KL, Roche PM, Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM (2009). Far-red fluorescent tags for protein imaging in living tissues. Biochem J 418 (3), 567–574

23. Martynova NY, Eroshkin FM, Ermolina LV, Ermakova GV, Korotaeva AL, Smurova KM, Gyoeva FK, Zaraisky AG (2008). The LIM-domain protein zyxin binds the homeodomain factor Xanf1/Hesx1 and modulates its activity in the anterior neural plate of Xenopus laevis embryo. Dev Dyn 237 (3), 736–749

24. Shcherbo D, Merzlyak EM, Chepurnykh TV, Fradkov AF, Ermakova GV, Solovieva EA, Lukyanov KA, Bogdanova EA, Zaraisky AG, Lukyanov S, Chudakov DM (2007). Bright far-red fluorescent protein for whole-body imaging. Nat Methods 4 (9), 741–746

25. Ermakova GV, Solovieva EA, Martynova NY, Zaraisky AG (2007). The homeodomain factor Xanf represses expression of genes in the presumptive rostral forebrain that specify more caudal brain regions. Dev Biol 307 (2), 483–497

26. Zaraisky AG (2007). Neural induction: New achievements and prospects. Mol Biol 41 (2), 173–186

27. Evdokimov AG, Pokross ME, Egorov NS, Zaraisky AG, Yampolsky IV, Merzlyak EM, Shkoporov AN, Sander I, Lukyanov KA, Chudakov DM (2006). Structural basis for the fast maturation of Arthropoda green fluorescent protein. EMBO Rep 7 (10), 1006–1012

28. Efimov VA, Birikh KR, Staroverov DB, Lukyanov SA, Tereshina MB, Zaraisky AG, Chakhmakhcheva OG (2006). Hydroxyproline-based DNA mimics provide an efficient gene silencing in vitro and in vivo. Nucleic Acids Res 34 (8), 2247–2257

29. Tereshina MB, Zaraisky AG, Novoselov VV (2006). Ras-dva, a member of novel family of small GTPases, is required for the anterior ectoderm patterning in the Xenopus laevis embryo. Development 133 (3), 485–494

30. Eroshkin FM, Ermakova GV, Bayramov AV, Zaraisky AG (2006). Multiple noggins in vertebrate genome: Cloning and expression of noggin2 and noggin4 in Xenopus laevis. Gene Expr Patterns 6 (2), 180–186

31. Zaraisky AG (2004). Xenopus embryos as a model to study the genetic mechanisms of brain development. Mol Biol (Mosk) 38 (1), 40–47

32. Bayramov AV, Martynova NY, Eroshkin FM, Ermakova GV, Zaraisky AG (2004). The homeodomain-containing transcription factor X-nkx-5.1 inhibits expression of the homeobox gene Xanf-1 during the Xenopus laevis forebrain development. Mech Dev 121 (12), 1425–1441

33. Martynova N, Eroshkin F, Ermakova G, Bayramov A, Gray J, Grainger R, Zaraisky A (2004). Patterning the forebrian: FoxA4a/Pintallavis and Xvent2 determine the posterior limit of Xanf1 expression in the neural plate. Development 131 (10), 2329–2338

34. Verkhusha VV, Kuznetsova IM, Stepanenko OV, Zaraisky AG, Shavlovsky MM, Turoverov KK, Uversky VN (2003). High stability of Discosoma DsRed as compared to Aequorea EGFP. Biochemistry 42 (26), 7879–7884

35. Novoselov VV, Alexandrova EM, Ermakova GV, Zaraisky AG (2003). Expression zones of three novel genes abut the developing anterior neural plate of Xenopus embryo. Gene Expr Patterns 3 (2), 225–230

36. Chudakov DM, Belousov VV, Zaraisky AG, Novoselov VV, Staroverov DB, Zorov DB, Lukyanov S, Lukyanov KA (2003). Kindling fluorescent proteins for precise in vivo photolabeling. Nat Biotechnol 21 (2), 191–194

37. Terskikh AV, Fradkov AF, Zaraisky AG, Kajava AV, Angres B (2002). Analysis of DsRed mutants: Space around the fluorophore accelerates fluorescence development. J Biol Chem 277 (10), 7633–7636

38. Eroshkin F, Kazanskaya O, Martynova N, Zaraisky A (2002). Characterization of cis-regulatory elements of the homeobox gene Xanf-1. Gene 285 (12), 279–286

39. Brockmann B, Smith MW, Zaraisky AG, Harrison K, Okada K, Kamiya Y (2001). Subcellular localization and targeting of glucocorticoid receptor protein fusions expressed in transgenic Arabidopsis thaliana. Plant Cell Physiol 42 (9), 942–951

40. Terskikh A, Fradkov A, Ermakova G, Zaraisky A, Tan P, Kajava AV, Zhao X, Lukyanov S, Matz M, Kim S, Weissman I, Siebert P (2000). 'Fluorescent timer': Protein that changes color with time. Science 290 (5496), 1585–1588

41. Lukyanov KA, Fradkov AF, Gurskaya NG, Matz MV, Labas YA, Savitsky AP, Markelov ML, Zaraisky AG, Zhao X, Fang Y, Tan W, Lukyanov SA (2000). Natural animal coloration can be determined by a nonfluorescent green fluorescent protein homolog. J Biol Chem 275 (34), 25879–25882

42. Alexandrova EM, Zaraisky AG (2000). Molecular mechanisms of early neurogenesis in vertebrates. Mol Biol 34 (4), 496–507

43. Matz MV, Fradkov AF, Labas YA, Savitsky AP, Zaraisky AG, Markelov ML, Lukyanov SA (1999). Fluorescent proteins from nonbioluminescent Anthozoa species. Nat Biotechnol 17 (10), 969–973

44. Ermakova GV, Alexandrova EM, Kazanskaya OV, Vasiliev OL, Smith MW, Zaraisky AG (1999). The homeobox gene, Xanf-1, can control both neural differentiation and patterning in the presumptive anterior neurectoderm of the Xenopus laevis embryo. Development 126 (20), 4513–4523

45. Kazanskaya OV, Ermakova GV, Pannese M, Boncinelli E, Zaraisky AG (1998). cDNA Cloning of Three New Homeobox-Containing Genes of the Anf Class from Human, Chicken, and Newt. Bioorg Khim 24 (3), 192–193

46. Bogdanova E, Matz M, Tarabykin V, Usman N, Shagin D, Zaraisky A, Lukyanov S (1998). Inductive interactions regulating body patterning in planarian, revealed by analysis of expression of novel gene scarf. Dev Biol 194 (2), 172–181

47. Vasiliev OL, Lukyanov SA, Belyavsky AV, Kazanskaya OV, Zaraisky AG (1997). A novel marker of early epidermal differentiation: cDNA subtractive cloning starting on a single explant of Xenopus laevis gastrula epidermis. Int J Dev Biol 41 (6), 877–882

48. Kazanskaya OV, Severtzova EA, Barth KA, Ermakova GV, Lukyanov SA, Benyumov AO, Pannese M, Boncinelli E, Wilson SW, Zaraisky AG (1997). Anf: A novel class of vertebrate homeobox genes expressed at the anterior end of the main embryonic axis. Gene 200 (12), 25–34

49. Lukyanov KA, Launer GA, Tarabykin VS, Zaraisky AG, Lukyanov SA (1995). Inverted terminal repeats permit the average length of amplified dna fragments to be regulated during preparation of cdna libraries by polymerase chain reaction. Anal Biochem 229 (2), 198–202

50. Zaraisky AG, Ecochard V, Kazanskaya OV, Lukyanov SA, Fesenko IV, Duprat AM (1995). The homeobox-containing gene XANF-1 may control development of the Spemann organizer. Development 121 (11), 3839–3847

51. Ecochard V, Cayrol C, Foulquier F, Zaraisky A, Duprat AM (1995). A novel TGF-β-like gene, fugacin, specifically expressed in the spemann organizer of Xenopus. Dev Biol 172 (2), 699–703

52. Zaraisky AG, Lukyanov SA, Vasiliev OL, Smirnov YV, Belyavsky AV, Kazanskaya OV (1992). A novel homeobox gene expressed in the anterior neural plate of the Xenopus embryo. Dev Biol 152 (2), 373–382

53. Belintsev BN, Beloussov LV, Zaraisky AG (1987). Model of pattern formation in epithelial morphogenesis. J Theor Biol 129 (4), 369–394

Scientific projects

1. - (January 6, 2019 — December 31, 2021). Zaraisky A.G.. Grant, RSF.

2. - (August 7, 2018 — August 7, 2021). Zaraisky A.G.. Grant, RFBR.